|
真菌多样性与进化研究专题组
|
发表论文
Lateral gene transfer occurring in haloarchaea: an interpretative imitation study Abstract Lateral gene transfer (LGT) plays an important pSY1 and pWL102, were then produced in media at role in the molecular evolution of haloarchaea. Polyethyl- strain-appropriate MGSCs. The four strains of haloarchaea ene glycol-mediated LGT in haloarchaea has been dem- were transformed successfully by both kinds of dsDNAs onstrated in the laboratory, yet few explanations have been with an efficiency of 102–103transformants per microgram put forward for the apparently common, natural occurrence dsDNA. The transformation under reduced salt concentra of plentiful plasmids within haloarchaeal cells. In this tion may be an imitation of natural LGT of dsDNA into study, LGT was induced in two genera of haloarchaea, haloarchaea when salinity in normally hypersaline enviHaloferaxandHalorubrum, by modification of salt con- ronments is altered by sudden introduction of fresh water centration of media-a factor that may vary naturally in for example, by rainfall, snow-melt, or flooding—providnative haloarchaeal habitat. Minimal growth salt concen- ing a reasonable interpretation for haloarchaea being nattrations (MGSCs) of four strains of haloarchaea from urally richer in plasmids than any other known organisms. these two genera were established, and transformations using two circular double-stranded DNAs (dsDNAs).
|
版权所有 Copyright © 2002-2025 中科院昆明植物研究所,All Rights Reserved 【滇ICP备05000394号】
地址：中国云南省昆明市蓝黑路132号 邮政编码：650201
点击这里联系我们
